𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Effect of the hydration state of supports before lyophilization on subtilisin-A activity in organic media

✍ Scribed by Juhan Kim; Byung-Gee Kim

Publisher
John Wiley and Sons
Year
1996
Tongue
English
Weight
674 KB
Volume
50
Category
Article
ISSN
0006-3592

No coin nor oath required. For personal study only.

✦ Synopsis

Subtilisin-A was colyophilized with various types of support materials, such as Amberlite IRC-50, Celite545, chitosan, DEAE-cellulose, DOWEX-1, zeolite, glass bead, and polystyrene. The colyophilized enzyme was used for the optical resolution of racemic l-phenylethylamine with 2,2,2-trifluoroethylbutyrate in 3-methyl-3-pentanol. The enzyme activity in organic media changed dramatically according to the hydration state of the support materials before lyophilization. This effect was especially marked with supports of high water capacity (aquaphilicity), such as chitosan and DEAE-cellulose. By hydrating these supports of high aquaphilicity prior to lyophilization, subtilisin-A activity in organic media increased ca. 4-8 times, depending upon the supports used. This result suggests that the hydration state of aquaphilic support materials for colyophilization is critical t o determining enzyme activity in organic solvents. 0 1996

πŸ“œ SIMILAR VOLUMES

Solvent effects on the catalytic activit
Solvent effects on the catalytic activity of subtilisin suspended in organic solvents
✍ Teresa CorrΓͺa de Sampaio; Rui B. Melo; Teresa F. Moura; Stefan Michel; Susana Ba πŸ“‚ Article πŸ“… 1996 πŸ› John Wiley and Sons 🌐 English βš– 907 KB

We studied a model transesterification reaction catalyzed by subtilisin Carlsberg suspended in toluene, n-hexane, diisopropyl ether, and mixtures of these solvents. To account for solvent effects due to differences in water partitioning between the enzyme and the bulk solvents, we measured water sor

Site-directed and random immobilization
Site-directed and random immobilization of subtilisin on functionalized membranes: Activity determination in aqueous and organic media
✍ S. Viswanath; J. Wang; L. G. Bachas; D. A. Butterfield; D. Bhattacharyya πŸ“‚ Article πŸ“… 1998 πŸ› John Wiley and Sons 🌐 English βš– 90 KB πŸ‘ 2 views

Kinetic comparisons have been made between a randomly immobilized and a site-specifically immobilized subtilisin BPNЈ on microfiltration membranes of varying hydrophilicities in both aqueous and organic media. Site-directed mutagenesis was employed to introduce a single cysteine into the amino acid

Stability of protease in organic solvent
Stability of protease in organic solvent: Structural identification by solid-state NMR of lyophilized papain before and after 1-propanol treatment and the corresponding enzymatic activities
✍ Teruhiko Matsubara; Risa Fujita; Shigeru Sugiyama; Katsuhiro Kawashiro πŸ“‚ Article πŸ“… 2006 πŸ› John Wiley and Sons 🌐 English βš– 131 KB πŸ‘ 1 views

## Abstract Lyophilized enzyme powder is often used in organic solvents. However, the enzymatic activity decreases during the reaction process. In the present study, the relation between structural stability and enzymatic activity in an organic solvent was investigated. ^13^C cross‐polarization mag