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Effect of immobilization support, water activity, and enzyme ionization state on cutinase activity and enantioselectivity in organic media

✍ Scribed by Pedro Vidinha; Neil Harper; Nuno M. Micaelo; Nuno M.T. Lourenco; Marco D.R. Gomes da Silva; Joaquim M.S. Cabral; Carlos A.M. Afonso; Claudio M. Soares; Susana Barreiros

Publisher
John Wiley and Sons
Year
2004
Tongue
English
Weight
553 KB
Volume
85
Category
Article
ISSN
0006-3592

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## Abstract Lipase from __Burkholderia cepacia__ (lipase BC) and lipase B from __Candida antarctica__ (CALB) show an increase of the transesterification activity in toluene (up to 2.4‐ and 1.7‐fold, respectively), when lyophilized with 18‐crown‐6. Nevertheless, the increase was observed only for lo