## Abstract Colyophilization or codrying of subtilisin Carlsberg with the crown ethers 18‐crown‐6, 15‐crown‐5, and 12‐crown‐4 substantially improved enzyme activity in THF, acetonitrile, and 1,4‐dioxane in the transesterification reactions of __N__‐acetyl‐L‐phenylalanine ethylester and 1‐propanol a
Effect of support material and enzyme pretreatment on enantioselectivity of immobilized subtilisin in organic solvents
✍ Scribed by Bernard Orsat; Gray J. Drtina; Michael G. Williams; Alexander M. Klibanov
- Publisher
- John Wiley and Sons
- Year
- 1994
- Tongue
- English
- Weight
- 485 KB
- Volume
- 44
- Category
- Article
- ISSN
- 0006-3592
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📜 SIMILAR VOLUMES
We developed an FTIR (Fourier transform infrared) methodology for quantitatively assessing the secondary structure of proteins suspended in nonaqueous media. This methodology was used to measure the percentages of ␣-helices and -sheets of subtilisin Carlsberg, prepared under different conditions, p
## Abstract Lipase from __Burkholderia cepacia__ (lipase BC) and lipase B from __Candida antarctica__ (CALB) show an increase of the transesterification activity in toluene (up to 2.4‐ and 1.7‐fold, respectively), when lyophilized with 18‐crown‐6. Nevertheless, the increase was observed only for lo