Transformation ofBacillus subtilisby electroporation
β Scribed by Michele Stephenson; Paul Jarrett
- Publisher
- Springer-Verlag
- Year
- 1991
- Tongue
- English
- Weight
- 280 KB
- Volume
- 5
- Category
- Article
- ISSN
- 0951-208X
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π SIMILAR VOLUMES
Bacillus brevis 47 was successfully and reproducibly transformed with pUB110 plasmid DNA by electroporation with an efficiency of 104 transformants per/2g of DNA. This represents a 10-fold improvement over the chemical transformation method previously used for this organism.
Transformation of intact cells of Bacillus licheniformis 5A2a with plasmids pLM6 (2.8 kb), pC194 (2.9 kb) and pCPa9 (7.1 kb) by electroporation resulted in 1.5 x 106 , 1.2 x 106 , and 5.2 x 104 transformants per ~g DNA, respectively. Transformation was possible with plasmid DNA, which was religated
The present study deals with the mechanism by which plasmid pCED3 interferes with the growth of Bacillus subtilis. Plasmid pCED3 was constructed from pUB110 and pBR322 and contains the lacZ gene attached to the B. subtilis tms promoter. Plasmid derivatives that contain mutations in the tms promoter