Transformation ofBacillus megateriumby electroporation
✍ Scribed by Alejandro Moro; Julio César Sánchez; Che Serguera
- Publisher
- Springer-Verlag
- Year
- 1995
- Tongue
- English
- Weight
- 135 KB
- Volume
- 9
- Category
- Article
- ISSN
- 0951-208X
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📜 SIMILAR VOLUMES
Bacillus brevis 47 was successfully and reproducibly transformed with pUB110 plasmid DNA by electroporation with an efficiency of 104 transformants per/2g of DNA. This represents a 10-fold improvement over the chemical transformation method previously used for this organism.
Transformation of intact cells of Bacillus licheniformis 5A2a with plasmids pLM6 (2.8 kb), pC194 (2.9 kb) and pCPa9 (7.1 kb) by electroporation resulted in 1.5 x 106 , 1.2 x 106 , and 5.2 x 104 transformants per ~g DNA, respectively. Transformation was possible with plasmid DNA, which was religated