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A highly efficient electroporation system for transformation ofBacillus licheniformis

✍ Scribed by Patrizia Brigidi; Edda Rossi; Giovanna Riccardi; Diego Matteuzzi

Publisher
Springer-Verlag
Year
1991
Tongue
English
Weight
251 KB
Volume
5
Category
Article
ISSN
0951-208X

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✦ Synopsis

Transformation of intact cells of Bacillus licheniformis 5A2a with plasmids pLM6 (2.8 kb), pC194 (2.9 kb) and pCPa9 (7.1 kb) by electroporation resulted in 1.5 x 106 , 1.2 x 106 , and 5.2 x 104 transformants per ~g DNA, respectively.

Transformation was possible with plasmid DNA, which was religated after restriction endonuclease digestions.

In addition, evidence is presented that indicates that B. licheniformis possesses DNA restriction and modification systems.

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