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Establishment of a highly efficient transformation system for pepper (Capsicum annuumL.)

✍ Scribed by D. Li; K. Zhao; B. Xie; B. Zhang; K. Luo


Publisher
Springer
Year
2003
Tongue
English
Weight
144 KB
Volume
21
Category
Article
ISSN
0721-7714

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A highly efficient electroporation syste
✍ Patrizia Brigidi; Edda Rossi; Giovanna Riccardi; Diego Matteuzzi πŸ“‚ Article πŸ“… 1991 πŸ› Springer-Verlag 🌐 English βš– 251 KB

Transformation of intact cells of Bacillus licheniformis 5A2a with plasmids pLM6 (2.8 kb), pC194 (2.9 kb) and pCPa9 (7.1 kb) by electroporation resulted in 1.5 x 106 , 1.2 x 106 , and 5.2 x 104 transformants per ~g DNA, respectively. Transformation was possible with plasmid DNA, which was religated