Transformation of the astaxanthin-producing yeastPhaffia rhodozyma
✍ Scribed by J. L. Adrio; M. Veiga
- Publisher
- Springer-Verlag
- Year
- 1995
- Tongue
- English
- Weight
- 402 KB
- Volume
- 9
- Category
- Article
- ISSN
- 0951-208X
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✦ Synopsis
This paper describes the genetic transformation of the astaxanthin-producing yeast Phafia rhodozyma with the cloning vector pGH-1. The plasmid replicates autonomously in this yeast, and the selection of transformants was possible by using both, the lJRA3 marker from Saccharomyces cerevisiae, and the kanamycin resistance (KmR) determinant from the bacterial transposon Tn903.
📜 SIMILAR VOLUMES
Distilled water and 0.02 molar citrate buffer pH 7.0, are suitable autolysing systems for the red yeast P. rk0d0z~a. Autolysis renders astaxanthin extractable from the yeast. Of six strains of the yeast tested, 67-484 was most susceptible to autolysis.
A new mutation strategy, which involves y-irradiation of cells followed by a selective enzymatic enrichment step, was worked out to obtain auxotrophic mutants from the astaxanthin-producing yeast P. rhodozymu. Under the optimized conditions described, different mutants suitable for strain improvemen