✦ LIBER ✦
A new mutation protocol for obtaining auxotrophic mutants of the yeastPhaffia rhodozyma
✍ Scribed by Zs. Palágyi; Á. Nagy; Cs. Vágvölgyi; L. Ferenczy
- Publisher
- Springer-Verlag
- Year
- 1995
- Tongue
- English
- Weight
- 160 KB
- Volume
- 9
- Category
- Article
- ISSN
- 0951-208X
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✦ Synopsis
A new mutation strategy, which involves y-irradiation of cells followed by a selective enzymatic enrichment step, was worked out to obtain auxotrophic mutants from the astaxanthin-producing yeast P. rhodozymu. Under the optimized conditions described, different mutants suitable for strain improvement were isolated. ATCC 24229 3.5 hours Arg-(2), Met-(3) 81 4 * Numbers of isolated mutants in parentheses. REFERENCES.