This paper describes the genetic transformation of the astaxanthin-producing yeast Phafia rhodozyma with the cloning vector pGH-1. The plasmid replicates autonomously in this yeast, and the selection of transformants was possible by using both, the lJRA3 marker from Saccharomyces cerevisiae, and the
Electrophoretic karyotype of the astaxanthin-producing yeastPhaffia rhodozyma
✍ Scribed by José L. Adrio; Mercedes López; Javier Casqueiro; Concepción Fernández; Manuel Veiga
- Publisher
- Springer-Verlag
- Year
- 1995
- Tongue
- English
- Weight
- 363 KB
- Volume
- 27
- Category
- Article
- ISSN
- 0172-8083
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A new mutation strategy, which involves y-irradiation of cells followed by a selective enzymatic enrichment step, was worked out to obtain auxotrophic mutants from the astaxanthin-producing yeast P. rhodozymu. Under the optimized conditions described, different mutants suitable for strain improvemen