Transformation of C3H/10T1/2 CL8 mouse embryo fibroblasts by ultraviolet irradiation and a phorbol ester
โ Scribed by MONDAL, SUKDEB; HEIDELBERGER, CHARLES
- Book ID
- 109698996
- Publisher
- Nature Publishing Group
- Year
- 1976
- Tongue
- English
- Weight
- 215 KB
- Volume
- 260
- Category
- Article
- ISSN
- 0028-0836
- DOI
- 10.1038/260710a0
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A cDNA clone of a rat cytochrome P450b gene was used to construct an expression vector driven by an SV40 promoter and containing a G418-resistance selectable marker. This bifunctional plasmid (pJRSL100) was transfected into the C3H 10T1/2CL8 mouse embryo fibroblast cell line. G418-resistant clones w
## Abstract In order to increase the sensitivity of the C3H/10T1/2 CL8 (10T1/2) cell transformation system, we increased the chemical exposure period to a total of 6 days (two consecutive 3โday exposures). Using this modified procedure, we transformed 10T1/2 cells with procarcinogens such as aflato