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Morphological transformation of C3H/10T1/2 CL8 cells by procarcinogens

✍ Scribed by Oshiro, Y. ;Balwierz, P. S.

Publisher
Wiley (John Wiley & Sons)
Year
1982
Weight
218 KB
Volume
4
Category
Article
ISSN
0192-2521

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✦ Synopsis

Abstract

In order to increase the sensitivity of the C3H/10T1/2 CL8 (10T1/2) cell transformation system, we increased the chemical exposure period to a total of 6 days (two consecutive 3‐day exposures). Using this modified procedure, we transformed 10T1/2 cells with procarcinogens such as aflatoxin B~1~, benz(a)anthracene, and 4‐nitroquinoline‐1‐oxide which have been negative in the standard 10T1/2 cell transformation assay. However, β‐naphthylamine was inconclusive and 2‐acetylamino‐fluorine was negative in this modified assay system. Our results demonstrate that a simple modification of the 10T1/2 cell transformation method can increase the sensitivity to some procarcinogens that require metabolic activation.

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