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Increased thymidine uptake by methylcholanthrene-treated C3H/10T1/2 cells

✍ Scribed by Fred Bairstow; Charles Heidelberger

Publisher
John Wiley and Sons
Year
1975
Tongue
French
Weight
371 KB
Volume
16
Category
Article
ISSN
0020-7136

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✦ Synopsis

Abstract

The uptake of ^3^H‐thymidine in post‐confluent cultures of methylcholanthrene‐transformed C3H/10T1/2 mouse embryo cells was markedly higher than in their non‐transformed counterparts. In a reconstruction experiment as few as 2% transformed cells could be detected by increased thymidine uptake. Measurements made at various times up to 110 days in multi‐chambered plates revealed that after 25 days methyl‐cholanthrene‐treated cultures incorporated significantly more thymidine than the acetone‐treated controls. This increased uptake correlated with the appearance of Type III transformed foci.

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