Quantitative analysis of the metabolism of benzo(a)pyrene by transformable C3H10T1/2CL8 mouse embryo fibroblasts
โ Scribed by Kenneth Rudo; Scott Ellis; B. J. Bryant; Kay Lawrence; Gaynelle Curtis; Helen Garland; Stephen Nesnow
- Book ID
- 112213268
- Publisher
- John Wiley and Sons
- Year
- 1986
- Tongue
- English
- Weight
- 779 KB
- Volume
- 6
- Category
- Article
- ISSN
- 0270-3211
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
A cDNA clone of a rat cytochrome P450b gene was used to construct an expression vector driven by an SV40 promoter and containing a G418-resistance selectable marker. This bifunctional plasmid (pJRSL100) was transfected into the C3H 10T1/2CL8 mouse embryo fibroblast cell line. G418-resistant clones w
## Abstract In order to increase the sensitivity of the C3H/10T1/2 CL8 (10T1/2) cell transformation system, we increased the chemical exposure period to a total of 6 days (two consecutive 3โday exposures). Using this modified procedure, we transformed 10T1/2 cells with procarcinogens such as aflato