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Transformation of Bacillus thuringiensis by electroporation

✍ Scribed by J. Mahillon; W. Chungjatupornchai; J. Decock; S. Dierickx; F. Michiels; M. Peferoen; H. Joos

Book ID
109314910
Publisher
John Wiley and Sons
Year
1989
Tongue
English
Weight
422 KB
Volume
60
Category
Article
ISSN
0378-1097

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✍ Schurter, Walter ;Geiser, Martin ;MathΓ©, DaniΓ¨le πŸ“‚ Article πŸ“… 1989 πŸ› Springer 🌐 English βš– 565 KB

Electroporation was used as a method to transform intact cells of Bacillus thuringiensis and B. cereus. With our optimized method a range of plasmid vectors could be transformed into strains of B. thuringiensis at frequencies of up to 10(7) transformants/micrograms DNA. This high frequency allows cl