𝔖 Bobbio Scriptorium
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Transformation of Bacillus thuringiensis by electroporation

✍ Scribed by Eileen J. Bone; David J. Ellar


Book ID
109314680
Publisher
John Wiley and Sons
Year
1989
Tongue
English
Weight
436 KB
Volume
58
Category
Article
ISSN
0378-1097

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Electroporation was used as a method to transform intact cells of Bacillus thuringiensis and B. cereus. With our optimized method a range of plasmid vectors could be transformed into strains of B. thuringiensis at frequencies of up to 10(7) transformants/micrograms DNA. This high frequency allows cl