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Transfer of yeast artificial chromosomes into mammalian cells and comparative study of their integrity

✍ Scribed by Samuel J.P. Gobin; Catherine Alcaïde-Loridan; Maria-Rosa Bono; Catherine Ottone; Ilya Chumakov; Rodney Rothstein; Marc Fellous

Book ID
116132965
Publisher
Elsevier Science
Year
1995
Tongue
English
Weight
752 KB
Volume
163
Category
Article
ISSN
0378-1119

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Transfer of yeast artificial chromosomes
Transfer of yeast artificial chromosomes from yeast to mammalian cells
✍ Clare Huxley; Andreas Gnirke 📂 Article 📅 1991 🏛 John Wiley and Sons 🌐 English ⚖ 729 KB

Human DNA can he cloned as yeast artificial chromosomes (YACs), each of which contains several hundred kilobases of human DNA. This DNA can be manipulated in the yeast host using homologous recombination and yeast selectable markers. In relatively few steps it is possible to make virtually any chang