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New vector for transfer of yeast artificial chromosomes to mammalian cells

✍ Scribed by David Markie; Jiannis Ragoussis; Gabriele Senger; Andrew Rowan; David Sansom; John Trowsdale; Denise Sheer; Walter F. Bodmer

Book ID
112501321
Publisher
Springer
Year
1993
Tongue
English
Weight
868 KB
Volume
19
Category
Article
ISSN
1572-9931

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Human DNA can he cloned as yeast artificial chromosomes (YACs), each of which contains several hundred kilobases of human DNA. This DNA can be manipulated in the yeast host using homologous recombination and yeast selectable markers. In relatively few steps it is possible to make virtually any chang