Transfer of yeast artificial chromosomes from yeast to mammalian cells

✍ Scribed by Clare Huxley; Andreas Gnirke

• Publisher: John Wiley and Sons
• Year: 1991
• Tongue: English
• Weight: 729 KB
• Volume: 13
• Category: Article
• ISSN: 0265-9247
• DOI: 10.1002/bies.950131009

✦ Synopsis

Human DNA can he cloned as yeast artificial chromosomes (YACs), each of which contains several hundred kilobases of human DNA. This DNA can be manipulated in the yeast host using homologous recombination and yeast selectable markers. In relatively few steps it is possible to make virtually any change in the cloned human DNA from single base pair changes to deletions and insertions. In order to study the function of the cloned DNA and the effects of the changes made in the yeast, the human DNA must be transferred back into mammalian cells. Recent experiments indicate that large genes can be transferred from the yeast host to mammalian cells in tissue culture and that the genes are transferred intact and are expressed. Using the same methods it may soon be possible to transfer YAC DNA into the mouse germ line so that the expression and function of genes cloned in YACs can be studied in developing and adult mammalian animals.