𝔖 Bobbio Scriptorium
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Transfection with Mu-DNA

✍ Scribed by Pieter van de Putte; Gerth C. Westmaas; Carel Wijffelman

Book ID
119009385
Publisher
Elsevier Science
Year
1977
Tongue
English
Weight
668 KB
Volume
81
Category
Article
ISSN
0042-6822

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πŸ“œ SIMILAR VOLUMES

Transfection of Escherichia coli by Mu D
Transfection of Escherichia coli by Mu DNA
✍ Kahmann, R. ;Kamp, D. ;Zipser, D. πŸ“‚ Article πŸ“… 1976 πŸ› Springer 🌐 English βš– 536 KB

Infectivity of Mu DNA was demonstrated in Ca+ +-treated Escherichia coli cells that lacked the nucleases Exo V and Endo I. The efficiency of transfection is about 10(-7) per phage equivalent. Infectivity is destroyed by denaturation of Mu DNA, and cannot be restored by renaturation.

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Mycobacterium smegmatis SN2 does not exhibit natural competence for the uptake of phage I3 DNA. Competence can artificially be induced by treatment with glycine or CaCI2, and the combination of both is even more effective. The efficiency of transfection can be improved by inclusion of protamine sulp