✦ LIBER ✦

Transfection ofMycobacterium smegmatisSN2 with mycobacteriophage I3 DNA

✍ Scribed by S. Sadashiva Karnik; K. P. Gopinathan

Book ID: 104770105
Publisher: Springer
Year: 1983
Tongue: English
Weight: 560 KB
Volume: 136
Category: Article
ISSN: 0302-8933
DOI: 10.1007/bf00425216

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✦ Synopsis

Mycobacterium smegmatis SN2 does not exhibit natural competence for the uptake of phage I3 DNA. Competence can artificially be induced by treatment with glycine or CaCI2, and the combination of both is even more effective. The efficiency of transfection can be improved by inclusion of protamine sulphate and heterologous RNA in the system. From 32p DNA uptake studies the major barrier for the entry of DNA has been found to be the complex cell wall. The efficiency of transfection calculated on the basis of fraction of D N A which has entered the cell is comparable to that of other bacterial systems. The phage development takes a longer time (7 h for one cycle) after transfection, as compared to infection (4 h).

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