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Transfection of Escherichia coli by Mu DNA

✍ Scribed by Kahmann, R. ;Kamp, D. ;Zipser, D.

Publisher: Springer
Year: 1976
Tongue: English
Weight: 536 KB
Volume: 149
Category: Article
ISSN: 0026-8925
DOI: 10.1007/bf00268534

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✦ Synopsis

Infectivity of Mu DNA was demonstrated in Ca+ +-treated Escherichia coli cells that lacked the nucleases Exo V and Endo I. The efficiency of transfection is about 10(-7) per phage equivalent. Infectivity is destroyed by denaturation of Mu DNA, and cannot be restored by renaturation.

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