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Transcription of insertion elements IS1 and IS2 in vitro

✍ Scribed by Besemer, Jürgen ;Molzberger, Christel


Book ID
105045409
Publisher
Springer
Year
1977
Tongue
English
Weight
878 KB
Volume
153
Category
Article
ISSN
0026-8925

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✍ Rak, Bodo 📂 Article 📅 1983 🏛 Springer 🌐 English ⚖ 621 KB

Presence of insertion element IS1 within an operon leads to absence of expression of genes distal to its integration site. This strong polar effect is observed irrespective of the orientation of IS1. Here I report on a mutant of E. coli K12 which allows turn-on of genes located distally to IS1. This

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✍ Nyman, Kate ;Ohtsubo, Hisako ;Davison, Daniel ;Ohtsubo, Eiichi 📂 Article 📅 1983 🏛 Springer 🌐 English ⚖ 275 KB

Total DNAs from twelve natural isolates of Escherichia coli from animals and humans were examined by hybridization with a probe for IS1. Considerable variation in copy number was found. In the case of two strains isolated from the same individual, one strain contained no copies of IS1 and the other,