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Distribution of insertion element IS1 in natural isolates of Escherichia coli

โœ Scribed by Nyman, Kate ;Ohtsubo, Hisako ;Davison, Daniel ;Ohtsubo, Eiichi


Book ID
104722783
Publisher
Springer
Year
1983
Tongue
English
Weight
275 KB
Volume
189
Category
Article
ISSN
0026-8925

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โœฆ Synopsis


Total DNAs from twelve natural isolates of Escherichia coli from animals and humans were examined by hybridization with a probe for IS1. Considerable variation in copy number was found. In the case of two strains isolated from the same individual, one strain contained no copies of IS1 and the other, much greater than 30. Evidence was also obtained for the existence of IS1-like elements (iso-IS1s) of greater than 15% sequence divergence relative to the IS1 from antibiotic resistance plasmid R100.


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The nucleotide sequence of the rpsE gene for the ribosomal protein S5 of a mutant of E. coli showing cold-sensitive growth revealed the presence of an insertion sequence, IS1, near the 3'-end of the gene. This mutant grows very slowly even at the "permissive temperature" (30 degrees C). At temperatu