## ABSTRACT The dependence of blastocyst development on the extracellular Na^+^/K^+^ ratio was investigated in an in vitro system. Hatching and outgrowth of mouse blastocysts was enhanced at Na^+^/K^+^ ratios between 3 and 10 compared to the ratio of about 25 typical for most culture media and seru
Toxic effects of Zn++and Cu++on mouse blastocysts in vitro
โ Scribed by L. J. Van Winkle; A. L. Campione
- Publisher
- Springer
- Year
- 1982
- Tongue
- English
- Weight
- 356 KB
- Volume
- 38
- Category
- Article
- ISSN
- 1420-682X
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
## Abstract The effect of inhibitors of RNA synthesis (Cordycepin, Actinomycin D) and protein synthesis (Cycloheximide) on the development and growth of mouse blastocysts in vitro was explored. Blastocysts exposed in vitro for 24 hours to 50 ฮผg/ml Cordycepin, 0.005 ฮผg/ml Actinomycin D, or 0.1 ฮผg/ml
Arginine, cystine, histidine, leucine and threonine were needed for outgrowth of the mouse blastocyst in vitro. Omission of lysine, methionine, phenylalanine, tryptophane and tyrosine from the culture medium markedly reduced blastocyst outgrowth, h u t did not inhibit it completely; while omission o
## Abstract The resumption of DNA synthesis in delayed implanting mouse embryos undergoing metabolic activation in vitro was examined. Blastocysts were recovered from ovariectomized mice, incubated for various intervals in basal Eagle's medium, exposed to ^3^Hโ thymidine, and prepared for light mic