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Towards Dark Quencher Based Real Time DNA Sequencing

✍ Scribed by Hohlbein, Johannes; Le Reste, Ludovic; Potapova, Olga; Joyce, Catherine; El-Sagheer, Afaf H.; Brown, Tom; Kapanidis, Achillefs N.

Book ID
122199079
Publisher
Biophysical Society
Year
2010
Tongue
English
Weight
57 KB
Volume
98
Category
Article
ISSN
0006-3495

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## Abstract **One's enough**: The direct observation of a DNA‐polymerase‐based “sequencing engine” using single‐molecule detection recently allowed single‐molecule sequencing by synthesis in real time. Nucleotides with a fluorescent marker at the 5′‐phosphate unit and zero‐mode waveguides are cruci