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Topologically Non-linked Circular Duplex DNA

✍ Scribed by Ken Biegeleisen


Publisher
Springer
Year
2002
Tongue
English
Weight
540 KB
Volume
64
Category
Article
ISSN
1522-9602

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Isolation of circular viral and compleme
✍ Marvin L. Bayne; Lawrence B. Dumas πŸ“‚ Article πŸ“… 1978 πŸ› Elsevier Science 🌐 English βš– 518 KB

A general method has been developed for the large scale isolation of intact, circular, single-stranded DNA molecules of each strand from supercoiled duplex DNA. The method involves the conversion of the supercoiled duplex DNA to singly nicked, relaxed duplex DNA; denaturation of the duplex DNA; sepa