Topological Links between Duplex DNA and a Circular DNA Single Strand
β Scribed by Heiko Kuhn; Vadim V. Demidov; Maxim D. Frank-Kamenetskii
- Publisher
- John Wiley and Sons
- Year
- 1999
- Tongue
- English
- Weight
- 117 KB
- Volume
- 38
- Category
- Article
- ISSN
- 0044-8249
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
A general method has been developed for the large scale isolation of intact, circular, single-stranded DNA molecules of each strand from supercoiled duplex DNA. The method involves the conversion of the supercoiled duplex DNA to singly nicked, relaxed duplex DNA; denaturation of the duplex DNA; sepa
## SYNOPSIS CD spectra and melting curves were collected for a 28 base-pair DNA fragment in the form of a DNA dumbbell (linked on both ends by T, single-strand loops) and the same DNA
Chemical modification of backbone structures has been an important strategy in designing oligonucleotides capable of improved antisense effects. However, altered backbone chemistry may also affect the binding of oligonucleotides to key cellular proteins, and thus may impact on the overall biological