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Topological Analysis of Plasmid Chromatin from Yeast and Mammalian Cells

✍ Scribed by Wilbur Tong; Olga I. Kulaeva; David J. Clark; Leonard C. Lutter

Book ID
116663177
Publisher
Elsevier Science
Year
2006
Tongue
English
Weight
911 KB
Volume
361
Category
Article
ISSN
0022-2836

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Human DNA can he cloned as yeast artificial chromosomes (YACs), each of which contains several hundred kilobases of human DNA. This DNA can be manipulated in the yeast host using homologous recombination and yeast selectable markers. In relatively few steps it is possible to make virtually any chang