## Abstract Recombinant human bone morphogenetic protein 2 (rhBMP‐2) has been widely employed for the induction of bone growth in animal models and in clinical trials. Since rhBMP‐2 is commercially available only to a very limited extent, we have prepared our own recombinant material for the curren
✦ LIBER ✦
Frequency and conditions of spontaneous plasmid transfer from E. coli to cultured mammalian cells
✍ Scribed by D. Heitmann; J.M. Lópes-Pila
- Publisher
- Elsevier Science
- Year
- 1993
- Tongue
- English
- Weight
- 881 KB
- Volume
- 29
- Category
- Article
- ISSN
- 0303-2647
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The E. coli F'lac plasmid was transferred from an Erwinia chrysanthemi Hfr8 donor to a multiply-auxotrophic, rifampicin-resistant Pseudomonas syringae pv. glycinea recipient. Transfer occurred at a frequency of approximately 10(-5)/donor. Stable transconjugants which were able to utilize lactose as