✦ LIBER ✦

Tissue plasminogen activator induced by dengue virus infection of human endothelial cells

✍ Scribed by Ying-Huey Huang; Huan-Yao Lei; Hsiao-Sheng Liu; Yee-Shin Lin; Shun-Hua Chen; Ching-Chuan Liu; Trai-Ming Yeh

Publisher: John Wiley and Sons
Year: 2003
Tongue: English
Weight: 143 KB
Volume: 70
Category: Article
ISSN: 0146-6615
DOI: 10.1002/jmv.10438

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✦ Synopsis

Abstract

Dengue hemorrhagic fever and dengue shock syndrome (DHF/DSS) are severe complications of dengue virus (DV) infection. However, the pathogenesis of hemorrhage induced by dengue virus infection is poorly understood. Since endothelial cells play a pivotal role in the regulation of hemostasis, we studied the effect of DV infection on the production of tissue plasminogen activator (tPA) and plasminogen activator inhibitor 1 (PAI‐1) in vitro using both primary isolated endothelial cells, human umbilical cord veins cells, and a human microvascular endothelial cell line. DV infection significantly induced the secretion of tPA but not PAI‐1 of human endothelial cells. In addition, tPA mRNA of endothelial cells was induced by DV as demonstrated by RT‐PCR. Antibody against IL‐6 but not control antibody inhibited DV‐induced tPA production of endothelial cells. Furthermore, a good correlation between sera levels of IL‐6 and tPA was found in DHF but not DF patients. These results suggest that IL‐6 can regulate DV‐induced tPA production of endothelial cells, which may play important roles in the pathogenic development of DHF/DSS. J. Med. Virol. 70:610–616, 2003. © 2003 Wiley‐Liss, Inc.

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