Effects of baicalein isolated from roots of Scutellaria baicalensis Georgi on interleukin 1β- and tumour necrosis factor α-induced tissue-type plasminogen activator and plasminogen activator inhibitor-1 production in cultured human umbilical vein endothelial cells
✍ Scribed by Yoshiyuki Kimura; Hiromichi Okuda; Kumi Yokoi (Hayashi); Nobutoshi Matsushita
- Publisher
- John Wiley and Sons
- Year
- 1997
- Tongue
- English
- Weight
- 85 KB
- Volume
- 11
- Category
- Article
- ISSN
- 0951-418X
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✦ Synopsis
The effects were examined of baicalein on tissue-type plasminogen activator (t-PA) and plasminogen activator inhibitor-1 (PAI-1) production stimulated by interleukin-1␣ (IL-1) and tumour necrosis factor-␣ (TNF-␣) in cultured human vein umbilical endothelial cells (HUVECs). IL-1 and TNF-␣ increased PAI-1 production, but they decreased t-PA production. Baicalein isolated from the roots of Scutellaria baicalensis Georgi, inhibited the PAI-1 production stimulated by IL-1 and TNF-␣, but it had no effect on the t-PA. In addition, protein kinase C (PKC) inhibitors such as H7 and sphingosine decreased the reduction of t-PA and the increase of PAI-1 by IL-1 and TNF-␣. On the other hand, dibutryl cAMP and forskolin had no effect on the t-PA reduction and PAI-1 increase by IL-1 and TNF-␣. Moreover, IL-1 and TNF-␣ did not cause [Ca 2+ ] elevation. These results suggest that the inhibitory action of baicalein on the PAI-1 production by both cytokines might be directly mediated through PKC inhibition.