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Human cytomegalovirus infection induces mRNA expression and secretion of plasminogen inhibitor type-1 in endothelial cells

✍ Scribed by Woodroffe, Salwa B.; Kuan, Siong

Publisher
John Wiley and Sons
Year
1998
Tongue
English
Weight
125 KB
Volume
55
Category
Article
ISSN
0146-6615

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✦ Synopsis

The aim of the study was to investigate whether infection of endothelial cells with human cytomegalovirus (HCMV) perturbs expression and production of plasminogen activator inhibitor type-1 (PAI-1). mRNA expression of PAI-1 was investigated by isolating total RNA from HCMVinfected and control cells, followed by Northern blotting and probing with 32 P-labelled PAI-1 probe. Sandwich ELISA was used to investigate PAI-1 production. HCMV induced the expression of PAI-1-mRNA at 2-5 days postinfection (maximum expression was at 3 days postinfection which was 40% higher than control). HCMV also induced secretion of PAI-1 at 2-5 days postinfection. These results indicate that infection of endothelial cells with HCMV disturbs PAI-1 expression and production in these cells.

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