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Tissue cholesterol preservation: Solubility of cholesterol digitonide in ethanol

✍ Scribed by Sterzing, Peter R. ;Scaletti, Joseph V. ;Napolitano, Leonard M.

Publisher: John Wiley and Sons
Year: 1970
Tongue: English
Weight: 279 KB
Volume: 168
Category: Article
ISSN: 0003-276X
DOI: 10.1002/ar.1091680409

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

The solubility of purified cholesterol digitonide in absolute and aqueous ethanols was investigated. The results indicate that preservation of cholesterol (or other 3‐B‐hydroxysterols) in tissues prepared for electron and light microscopy by digitonin‐containing fixatives may not be quantitative when ethanol and, in particular, absolute ethanol, is used for dehydration.

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