## Abstract This work presents measurements of time‐resolved fluorescence microscopy of hematoporphyrin‐derivative (HpD) in single cells of mice tissue (both tumor and normal cells), in HeLa cells, and in solution. The measurements were performed using a pulsed‐laser microfluorometer with high spat
Time-resolved fluorescence spectroscopy of hematoporphyrin derivative in micelles
✍ Scribed by R. Cubeddu; R. Ramponi; G. Bottiroli
- Publisher
- Elsevier Science
- Year
- 1986
- Tongue
- English
- Weight
- 350 KB
- Volume
- 128
- Category
- Article
- ISSN
- 0009-2614
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✦ Synopsis
Time-resolved fluorescence spectroscopy was performed on hematoporphyrin derivative in buffer at different concentrations of surfactants. New molecular species were detected, with fluorescence decay time constants of -0.7 and ~~2.7 ns and emission peaks at 630 and 675 nm, respectively. Deawegation effects were observed, mainly in the presence of ationic micelles.
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Irradiation of an aqueous solution of hematoporphyrin causes the formation of the stable photoproduct which may play an important role in cancer therapy. The spectra and kinetic characteristics of the absorption changes of hematoporphyrins and their photoproducts were measured by a picosecond pump a