Three-Dimensional Protein NMR TROSY-Type 15N-resolved 1HN–1HN NOESY Spectra with Diagonal Peak Suppression

✍ Scribed by Axel Meissner; Ole Winneche Sørensen

• Publisher: Elsevier Science
• Year: 2000
• Tongue: English
• Weight: 94 KB
• Volume: 142
• Category: Article
• ISSN: 1090-7807
• DOI: 10.1006/jmre.1999.1961

✦ Synopsis

An earlier two-dimensional NOESY experiment with diagonal peak suppression in the 1 H N -1 H N region is extended to three dimensions by including 15 N evolution while maintaining the TROSY approach throughout. The technique suppresses all anti-TROSY resonances by appropriate pulse sequence elements and for large molecules at high fields possible semi-and anti-TROSY artifacts are further suppressed by virtue of much shorter transverse relaxation times for these components. The new technique is demonstrated using an 15 N-labeled protein sample, RAP 17-97 (N-terminal domain of ␣2-macroglobulin Receptor Associated Protein), in H 2 O at 500 MHz.