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Suppression of Diagonal Peaks in TROSY-Type 1H NMR NOESY Spectra of 15N-Labeled Proteins

✍ Scribed by Axel Meissner; Ole Winneche Sørensen

Publisher
Elsevier Science
Year
1999
Tongue
English
Weight
86 KB
Volume
140
Category
Article
ISSN
1090-7807

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✦ Synopsis

A novel method for suppression of diagonal peaks in the amide region of NOESY NMR spectra of 15 N-labeled proteins is presented. The method is particularly useful for larger proteins at high magnetic fields where interference between dipolar and chemical shift anisotropy relaxation mechanisms results in large TROSY effects, i.e., large differences in 1 H N linewidths depending on the spin state of attached 15 N nuclei. In this limit the new TROSY NOESY method does not compromise sensitivity. It is demonstrated using a perdeuterated 15 N-labeled protein sample, Neural Cell Adhesion Molecule 213-308 (NCAM) from rat, in H 2 O at 800 MHz.

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