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Enhanced diagonal peak suppression in three-dimensional TROSY-type15N-resolved1HN1HNNOESY spectra

✍ Scribed by Meissner, Axel ;Sørensen, Ole Winneche


Publisher
John Wiley and Sons
Year
2001
Tongue
English
Weight
167 KB
Volume
14
Category
Article
ISSN
1043-7347

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Three-Dimensional Protein NMR TROSY-Type
✍ Axel Meissner; Ole Winneche Sørensen 📂 Article 📅 2000 🏛 Elsevier Science 🌐 English ⚖ 94 KB

An earlier two-dimensional NOESY experiment with diagonal peak suppression in the 1 H N -1 H N region is extended to three dimensions by including 15 N evolution while maintaining the TROSY approach throughout. The technique suppresses all anti-TROSY resonances by appropriate pulse sequence elements

Suppression of Diagonal Peaks in TROSY-T
✍ Axel Meissner; Ole Winneche Sørensen 📂 Article 📅 1999 🏛 Elsevier Science 🌐 English ⚖ 86 KB

A novel method for suppression of diagonal peaks in the amide region of NOESY NMR spectra of 15 N-labeled proteins is presented. The method is particularly useful for larger proteins at high magnetic fields where interference between dipolar and chemical shift anisotropy relaxation mechanisms result