Three-dimensional 87Rb imaging of isolated pig hearts: Effects of regional ischemia

✍ Scribed by Dr. Valery V. Kupriyanov; Jie Shen; Bozena Kuzio; Jiankang Sun; Roxanne Deslauriers

• Publisher: John Wiley and Sons
• Year: 1998
• Tongue: English
• Weight: 604 KB
• Volume: 40
• Category: Article
• ISSN: 0740-3194
• DOI: 10.1002/mrm.1910400202

✦ Synopsis

Abstract

The aim of this work was to investigate whether 1) Rb uptake is reduced in ischemic myocardium, and 2) ^87^Rb three‐dimensional (3D) imaging can detect the ischemic area. Hearts of domestic pigs (n = 8, 20–30 kg) were perfused retrogradely with Krebs‐Henseleit buffer in a 7‐T, 40‐cm horizontal bore magnet interfaced with Bruker MSLX spectrometer. Control (C) and ischemic (I) (45 min ligation of the left anterior descending coronary artery (LAD)) hearts were loaded with Rb^+^ by perfusion with a Rb^+^‐containing solution (2–4.7 m__M__, 30–100% of K^+^ substitution) for 35 min and ^87^Rb (C) or ^31^P (ischemic area in I) spectra were acquired. After mechanical arrest with 0.6–0.9 m__M__ lidocaine, ^87^Rb images (7 min each, 1 cm^3^ resolution) were acquired (30–40 min) in the presence of Rb^+^‐containing perfusate. Subsequently, the hearts were stained with Evans blue (EB) and samples taken for measurements of Rb^+^ content. In the Group C, distribution of Rb^+^ in the left ventricle and the intensities of the 3D ^87^Rb images were uniform. In the ischemic area (Group I), verified by the lack of EB staining and changes in ^31^P spectra, the images showed a reduced intensity, which corresponded to decreased Rb^+^ content (33 ± 11% of the normal). Thus, ^87^Rb imaging reveals damaged cells detecting reduced Rb^+^ content in the ischemic area.