Imaging of ischemia and infarction in blood-perfused pig hearts using 87Rb MRI

✍ Scribed by V.V. Kupriyanov; B. Xiang; J. Sun; O. Jilkina; B. Kuzio

• Publisher: John Wiley and Sons
• Year: 2002
• Tongue: English
• Weight: 891 KB
• Volume: 49
• Category: Article
• ISSN: 0740-3194
• DOI: 10.1002/mrm.10338

✦ Synopsis

Abstract

^87^Rb‐MRI was used to measure Rb^+^ uptake in blood‐perfused pig hearts during complete occlusion (120 and 70 min) of the left anterior descending artery (LAD) and subsequent reperfusion (120 and 170 min). The Rb^+^ uptake rate and maximal Rb image intensity during 120‐min occlusion were significantly lower in the ischemic anterior left ventricular (LV) wall (0.35 ± 0.14%/min and 26 ± 4.7%) relative to those in the remote posterior LV wall (2.43 ± 0.33%/min and 98 ± 10%). Reperfusion after 120 and 70 min of occlusion resulted in formation of damaged areas, which had 40 ± 4 and 73 ± 10% of the ^87^Rb image intensity observed in the remote posterior wall. The infarct sizes determined histologically by triphenyltetrazolium chloride staining were 9.6 ± 3.4 and 5.6 ± 4.6% of the total ventricular mass (LV + RV) in the 120‐ and 70‐min occlusion groups, respectively. The sizes determined by MRI were 13.1 ± 2 and 2.8 ± 4.3% of the total number of pixels, respectively. The Rb^+^ uptake in the anterior wall during 120‐min occlusion was somewhat lower than that previously observed in crystalloid‐perfused hearts. It is concluded that blood does not interfere with the ability of ^87^Rb MRI to detect ischemic and infarct areas. Magn Reson Med 49:99–107, 2003. © 2003 Wiley‐Liss, Inc.