Lipids of human erythrocytes have been separated by silicic acid column chromatography, one-dimensional chromatography on silicic acid impregnated paper, or thin-layer chromatography (TLC), and combinations of silicic acid column chromatography with one-dimensional paper chromatography or TLC. The t
Thin-layer chromatography of lipids in adenovirus-treated human erythrocytes
✍ Scribed by Anna Lengyel; MÁrta Klembala; I. Nász
- Book ID
- 105134584
- Publisher
- Springer Vienna
- Year
- 1973
- Tongue
- English
- Weight
- 609 KB
- Volume
- 43
- Category
- Article
- ISSN
- 1432-8798
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A method for quantitative determination ofacidic phospholipids by thin-layer chromatography (TLC) followed by densitometry is described. The total lipids were separated into neutral and acidic fractions by DEAE-Sephadex column chromatography. A clear-cm separation of acidic phospholipids was achieve
The estimation of cholesterol in tissue lipid extracts is often complicated by the presence of substances which interfere with the calorimetric methods generally used. In these circumstances cholesterol is usually precipitated as the digitonide, and can then be estimated in the accustomed way. Separ