Thin-layer chromatography-densitometry of minor acidic phospholipids: Application to lipids from erythrocytes, liver, and kidney

✍ Scribed by Megumi Saito; Yasukazu Tanaka; Susumu Ando

• Publisher: Elsevier Science
• Year: 1983
• Tongue: English
• Weight: 1010 KB
• Volume: 132
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(83)90023-4

✦ Synopsis

A method for quantitative determination ofacidic phospholipids by thin-layer chromatography (TLC) followed by densitometry is described. The total lipids were separated into neutral and acidic fractions by DEAE-Sephadex column chromatography. A clear-cm separation of acidic phospholipids was achieved by high-performance TLC with a solvent system of chloroform/ acetone/acetic acid/formic acid/water (60/60/4/ 10/3). Each phospholipid band was quantitated by densitometry with the use. of an internal standard. The lipid compositions of sheep and mouSe erythrocytes and of rat liver and kidney were determined by the present method.