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Thin-layer chromatographic separation of intermediates of the pyridine nucleotide cycle

✍ Scribed by John T. Heard Jr.

Publisher: Elsevier Science
Year: 1983
Tongue: English
Weight: 281 KB
Volume: 130
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(83)90667-x

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✦ Synopsis

A rapid thin-layer chromatographic procedure for separation of the compounds comprising the intermediates in the salvage pathway known as the pyridine nucleotide cycle plus quinolinic acid and the reduced forms of nicotinamide adenine dinucleotide and nicotinamide adenine dinucleotide phosphate is described. The method utilizes silica gel high-performance thin-layer plates and a mobile phase of methanol, tetrabutylammonium hydroxide, and acetonitrile. The time required for analysis is greatly reduced and results in greater than 96% purity of each migrating compound.

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