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Thin-layer chromatographic separation of DNS-amino acids

✍ Scribed by D. Morse; B.L. Horecker

Publisher
Elsevier Science
Year
1966
Tongue
English
Weight
311 KB
Volume
14
Category
Article
ISSN
0003-2697

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✦ Synopsis

The use of fluorescent 1-dimethylaminonaphthalene-5-sulfonyl chloride (DNS-Cl)?

as a sensitive reagent for N-terminal analysis and sequence determinations on peptides has been described by Gray and Hartley (1, 2). Although this technique permits sequence determination with millimicromolar quantities of peptide, it has the disadvantage of a requirement for a specialized electrophoresis apparatus, owing to the high solubility of the DNS-amino acids in the conventional organic liquid coolants. We have now developed a procedure based on thin-layer chromatography which permits the resolution of the DNS-amino acids on plates coated with silica gel G. The method is very sensitive, rapid, and reproducible, and the equipment is readily assembled and is trouble-free.

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