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Thin-layer Chromatographic Determination of Catecholamines in Rat Plasma

✍ Scribed by G. Alemany; M. Akaârir; C. Rosselló; A. Gamundi

Publisher
John Wiley and Sons
Year
1996
Tongue
English
Weight
215 KB
Volume
10
Category
Article
ISSN
0269-3879

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✦ Synopsis

A TLC method for the quantitative determination of epinephrine, norepinephrine and dopamine in rat plasma is described. After deprotenization, catecholamines were adsorbed on acid-alumina and acetylated. The acetyl derivatives were extracted using a C18 minicolumn, resolved on HPTLC plates and quantitated by fluorescence densitometry at 415 nm, using isoprenaline as internal standard.

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