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Thickness and density of protein films by optical mixing spectroscopy

โœ Scribed by E. E. Uzgiris; H. P. M. Fromageot

Publisher
Wiley (John Wiley & Sons)
Year
1976
Tongue
English
Weight
405 KB
Volume
15
Category
Article
ISSN
0006-3525

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โœฆ Synopsis

Abstract

The adsorption of protein films on polystyrene latex spheres was studied by optical mixing spectroscopy. With this technique, we show that both the hydrodynamic thickness of protein films and their optical density can be measured. Thus, we found that films of the glycoproteins isolated from the human erythrocyte membrane were four times as thick as films of either human serum albumin or bovine serum albumin for about the same surface coverage. This result suggests an endโ€on orientation for the adsorbed glycoprotein molecules, which is consistent with the model proposed by others for the orientation of these molecules at the surface of the red blood cell itself.

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