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Thiamine increases the specific activity of human liver branched chain α-ketoacid dehydrogenase

✍ Scribed by DANNER, DEAN J.; DAVIDSON, EUGENE D.; ELSAS., LOUIS J.


Book ID
109695019
Publisher
Nature Publishing Group
Year
1975
Tongue
English
Weight
252 KB
Volume
254
Category
Article
ISSN
0028-0836

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A new method using hydrophobic interaction chromatography on phenyl-Sepharose was developed to purify branched chain Lu-ketoacid dehydrogenase complex from commercially available frozen rat liver. Yields of greater than 50% were routinely achieved. The purified enzyme, composed of Elc~, El& and E2 s