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Activation of branched-chain α-ketoacid dehydrogenase in isolated hepatocytes by branched-chain α-ketoacids

✍ Scribed by Amy C. Han; Gary W. Goodwin; Ralph Paxton; Robert A. Harris


Book ID
115707392
Publisher
Elsevier Science
Year
1987
Tongue
English
Weight
881 KB
Volume
258
Category
Article
ISSN
0003-9861

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Purification of branched chain α-ketoaci
✍ Yoshiharu Shimomura; Ralph Paxton; Takayuki Ozawa; Robert A. Harris 📂 Article 📅 1987 🏛 Elsevier Science 🌐 English ⚖ 452 KB

A new method using hydrophobic interaction chromatography on phenyl-Sepharose was developed to purify branched chain Lu-ketoacid dehydrogenase complex from commercially available frozen rat liver. Yields of greater than 50% were routinely achieved. The purified enzyme, composed of Elc~, El& and E2 s