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Thermostability of three pectinesterase isoenzymes in tomato fruit

✍ Scribed by B. Laratta; R. Loiudice; A. Giovane; L. Quagliuolo; L. Servillo; D. Castaldo


Publisher
Elsevier Science
Year
1995
Tongue
English
Weight
413 KB
Volume
52
Category
Article
ISSN
0308-8146

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Disulfide bridges in tomato pectinestera
✍ Oskar MarkoviΔ‰; Hans JΓΆrnvall πŸ“‚ Article πŸ“… 1992 πŸ› Cold Spring Harbor Laboratory Press 🌐 English βš– 471 KB

## Abstract Analysis of tomato pectinesterase by carboxymethylation, with and without reduction, shows that the enzyme has two intrachain disulfide bridges. Analysis of fragments obtained from the native enzyme after digestion with pepsin identified bridges connecting Cys‐98 with Cys‐125, and Cys‐1