Purification and properties of the major pectinesterases in lemon fruits (Citrus limon)

The use of continuous-flow electrophores

The use of continuous-flow electrophoresis to remove pectin in the purification of the minor pectinesterases in lemon fruits (citrus limon)

✍ Helen M Macdonald; Roger Evans; Wendy J Spencer 📂 Article 📅 1994 🏛 John Wiley and Sons 🌐 English ⚖ 537 KB

## Abstract Continuous‐flow electrophoresis was used to separate pectin and coloured substances from extracts of lemon pectinesterase. Two minor lemon pectinesterases were subsequently separated from two major pectinesterases, using chromatography on Sephadex G‐75 and CM‐Sephadex C‐50. One minor pe